Active Recombinant Human ALK, GST-His Tag

• Cat.No.: ALK-92H
• Product Overview: Human ALK wt, internal fragment, amino acids L1066-S1437 (as in NCBI/Protein entry NP_004295.2), N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His&GST
• ProteinLength: 1066-1437 a.a.
• Description: This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer.
• Bio-activity: Specific activity: 93 pmol/μg×min
• Molecular Mass: 70,459 Da
• AA Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD PMGHHHHHHG RDSLEVLFQG PLAMVLQAMQ MELQSPEYKL SKLRTSTIMT DYNPNYCFAG KTSSISDLKE VPRKNITLIR GLGHGAFGEV YEGQVSGMPN DPSPLQVAVK TLPEVCSEQD ELDFLMEALI ISKFNHQNIV RCIGVSLQSL PRFILLELMA GGDLKSFLRE TRPRPSQPSS LAMLDLLHVA RDIACGCQYL EENHFIHRDI AARNCLLTCP GPGRVAKIGD FGMARDIYRA SYYRKGGCAM LPVKWMPPEA FMEGIFTSKT DTWSFGVLLW EIFSLGYMPY PSKSNQEVLE FVTSGGRMDP PKNCPGPVYR IMTQCWQHQP EDRPNFAIIL ERIEYCTQDP DVINTALPIE YGPLVEEEEK VPVRPKDPEG VPPLLVSQQA KREEERS
• Storage: -80°C. Avoid repeated freeze-thaw cycles!
• Concentration: 0.301 µg/µl (Bradford method using BSA as standard protein)
• Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol

Gene Information

• Gene Name: ALK anaplastic lymphoma receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: ALK
• Synonyms: ALK; CD246; NBLST3; anaplastic lymphoma receptor tyrosine kinase; ALK tyrosine kinase receptor; CD246 antigen; mutant anaplastic lymphoma kinase; NP_004295.2; EC 2.7.10.1
• Gene ID: 238
• mRNA Refseq: NM_004304
• Protein Refseq: NP_004295
• MIM: 105590
• UniProt ID: Q9UM73
• Chromosome Location: 2p23
• Pathway: Non-small cell lung cancer
• Function: ATP binding; NF-kappaB-inducing kinase activity; protein binding