Active Recombinant Human AKT2, His-tagged

• Cat.No.: AKT2-163H

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB(Akt1), PKB(Akt2), and PKB(Akt3). PKB/Akt is activated in cells exposed to diverse stimuli such as hormones, growth factors, and extracellular matrix components. The Thr-308 residue in the kinase domain and Ser-473 residue in the tail domain of Akt1 need to be phosphorylated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and 3-phosphoinositide-dependent protein kinase-2 (PDK2), respectively, for its maximal activation. The corresponding phosphorylation sites in Akt2 (Thr-309 and Ser-474) and Akt3 (Thr-305 and Ser- 472) have been identified, and Akt2 and Akt3 appear also to be regulated by PDK1 and PDK2. A limited number of studies of the expression pattern of the three Akt isoforms reveal Akt1 and Akt2 are ubiquitous, whereas Akt3 is expressed predominantly in brain, heart, and kidney. The activation mechanism remains to be fully characterized but occurs downstream of phosphoinositide 3-kinase (PI-3K). PI-3K generates phosphatidylinositol-3,4,5 trisphosphate (PIP(3)), lipid second messenger essential for the translocation of PKB/Akt to the plasma membrane where it is phosphorylated and activated by phosphoinositidedependent kinase-1 (PDK-1) and possibly other kinases. PKB/Akt phosphorylates and regulates the function of many cellular proteins involved in processes that include metabolism, apoptosis, and proliferation. The recombinant human Akt2 kinase (PKB) was expressed in Sf9 insect cells and purified by Ni-NTA agarose chromatography. The sequence based calculated molecular weight is 60.4 kDa.
• Form: Supplied in 50 mM Tris-HCl pH 8.5, 100 mM NaCl, 1 mM DTT, and 50% glycerol.
• Activity: > 4,000 U/mg (1 Unit is defined as 1 picomole phosphate transferred to synthetic peptide RPRAATF per min at 30 °C). N-terminal His-tagged full-length protein.
• Purity: > 95% by SDS-PAGE.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles

Gene Information

• Gene Name: AKT2 v-akt murine thymoma viral oncogene homolog 2 [ Homo sapiens ]
• Synonyms: AKT2; v-akt murine thymoma viral oncogene homolog 2; PKBB; PRKBB; PKBBETA; RAC-BETA; AKT2 kinase; rac protein kinase beta; Murine thymoma viral (v-akt) homolog-2; EC 2.7.11.1; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; Protein kinase Akt-2; Protein kinase B, beta; PKB beta
• Gene ID: AKT2
• mRNA Refseq: NM_001626
• Protein Refseq: NP_001617
• MIM: 164731
• UniProt ID: P31751
• Chromosome Location: 19q13.1-q13.2
• Pathway: Acute myeloid leukemia; Adipocytokine signaling pathway; Apoptosis; B cell receptor signaling pathway; Chemokine signaling pathway; Chronic myeloid leukemia; Colorectal cancer; Endometrial cancer; ErbB signaling pathway; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; Glioma; Insulin signaling pathway; Jak-STAT signaling pathway; MAPK signaling pathway; Melanoma; Neurotrophin signaling pathway; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Progesterone-mediated oocyte maturation; Prostate cancer; Renal cell carcinoma; Small cell lung cancer; T cell receptor signaling pathway; Tight junction; Toll-like receptor signaling pathway; VEGF signaling pathway; mTOR signaling pathway
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity