Recombinant Human AKT2, His-tagged

• Cat.No.: AKT2-165H
• Product Overview: Recombinant full-length human AKT2 was co-expressed with PDK1 by baculovirus infection in Sf9 insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
• Molecular Mass: 57 kDa
• Specific Activity: 7 pmol/min/µg. The enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate at 30 oC for 1 hour.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Purity: ≥90%
• Fomulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, 20% glycerol, and 3 mM DTT.
• Stability: >6 months at –80°C
• OfficialSymbol: AKT2

Gene Information

• Gene Name: AKT2 v-akt murine thymoma viral oncogene homolog 2 [ Homo sapiens ]
• Synonyms: AKT2; v-akt murine thymoma viral oncogene homolog 2; PKBB; PRKBB; PKBBETA; RAC-BETA; AKT2 kinase; rac protein kinase beta; Murine thymoma viral (v-akt) homolog-2; EC 2.7.11.1; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; Protein kinase Akt-2; Protein kinase B, beta; PKB beta
• Gene ID: 208
• mRNA Refseq: NM_001626
• Protein Refseq: NP_001617
• MIM: 164731
• UniProt ID: P31751
• Chromosome Location: 19q13.1-q13.2
• Pathway: Acute myeloid leukemia; Adipocytokine signaling pathway; Apoptosis; B cell receptor signaling pathway; Chemokine signaling pathway; Chronic myeloid leukemia; Colorectal cancer; Endometrial cancer; ErbB signaling pathway
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity