Active Recombinant Human AKR1B1, His-tagged

• Cat.No.: AKR1B1-9156H
• Product Overview: Recombinant human AKR1B1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Description: Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
• Bio-activity: Specific activity: approximately 0.2-0.9 units/mg (please enquire for specific batch value).Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of1 micromole NADPH/min at 25C. Specific activity was expressed in units/mg of protein.
• Molecular Mass: 35.8 kDa (316aa), confirmed by MALDI-TOF
• Endotoxin: Less than 0.1 ng/μg (IEU/μg) of recombinant human Aldose reductase by the LAL method
• Purity: More than 95%, as determined by SDS-PAGE
• Stability: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: AKR1B1 aldo-keto reductase family 1, member B1 (aldose reductase) [ Homo sapiens ]
• Official Symbol: AKR1B1
• Synonyms: AKR1B1; aldo-keto reductase family 1, member B1 (aldose reductase); ALDR1; aldose reductase; AR; aldehyde reductase 1; low Km aldose reductase; Lii5-2 CTCL tumor antigen; aldo-keto reductase family 1 member B1; ADR; ALR2; MGC1804
• Gene ID: 231
• mRNA Refseq: NM_001628
• Protein Refseq: NP_001619
• MIM: 103880
• UniProt ID: P15121
• Chromosome Location: 7q35
• Pathway: Fructose and mannose metabolism, organism-specific biosystem; Fructose and mannose metabolism, conserved biosystem; Galactose metabolism, organism-specific biosystem; Galactose metabolism, conserved biosystem; Glycerolipid metabolism, organism-specific biosystem; Glycerolipid metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: alditol:NADP+ 1-oxidoreductase activity; aldo-keto reductase (NADP) activity; electron carrier activity; glyceraldehyde oxidoreductase activity; oxidoreductase activity