Active Recombinant Human ADAM10 Protein, His-tagged

• Cat.No.: ADAM10-924H
• Product Overview: Recombinant Human ADAM10 (Accession # O14672) Thr214-Glu672, fused with a C-terminal 10-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf21 Insect Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Thr214
• Form: Supplied as a 0.2 µm filtered solution in MES, NaCl, ZnCl2,Glycerol and Brij-35.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2.The specific activity is >20 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human ADAM10, His-tagged has a calculated MW of 52 kDa. In SDS-PAGE migrates as 60 kDa, reducing conditions.
• Protein length: Thr214-Glu672
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ADAM10 ADAM metallopeptidase domain 10 [ Homo sapiens ]
• Official Symbol: ADAM10
• Synonyms: ADAM10; ADAM metallopeptidase domain 10; a disintegrin and metalloproteinase domain 10; disintegrin and metalloproteinase domain-containing protein 10; CD156c; HsT18717; kuz; MADM; CDw156; ADAM 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10; AD10
• Gene ID: 102
• mRNA Refseq: NM_001110
• Protein Refseq: NP_001101
• MIM: 602192
• UniProt ID: O14672
• Chromosome Location: 15q2