Active Recombinant Human ACVRL1 protein, hFc-tagged

• Cat.No.: ACVRL1-768H
• Product Overview: Recombinant Human ACVRL1 protein(NP_000011.2)(Met1-Gln118), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured by its ability to bind Human ENG-Fc in functional Elisa.2. Measured by its ability to inhibit BMP9 induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. The ED50 for this effect is typically 1-6 ng/mL in the presence of 2 ng/mL of recombinant human BMP9.
• Molecular Mass: The mature recombinant human ALK1/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer comprises 335 amino acids and has a predicted molecular mass of 37.4 kDa. As a result of glycosylation, the recombinant monomer migrates as an approximately 45-50 kDa protein in SDS-PAGE under reducing conditions.
• Protein length: Met1-Gln118
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ACVRL1 activin A receptor type II-like 1 [ Homo sapiens ]
• Official Symbol: ACVRL1
• Synonyms: ACVRL1; activin A receptor type II-like 1; ACVRLK1, ORW2; serine/threonine-protein kinase receptor R3; ALK1; HHT; HHT2; activin receptor-like kinase 1; TGF-B superfamily receptor type I; activin A receptor, type II-like kinase 1; ORW2; SKR3; ALK-1; TSR-I; ACVRLK1
• Gene ID: 94
• mRNA Refseq: NM_000020
• Protein Refseq: NP_000011
• MIM: 601284
• UniProt ID: P37023