Active Recombinant Human ACE2 Protein, FLAG-tagged, Biotinylated

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Protein Length: 18-740
• Description: The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 is known to be expressed in various human organs, and its organ- and cell-specific expression suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2, the latter is the causative agent of coronavirus disease-2019 (COVID-19). Multiple splice variants have been found for this gene and the dACE2 (or MIRb-ACE2) splice variant has been found to be interferon inducible.
• Bio-activity: ACE2 Human, HEK293 Recombinant binds with high affinity to the Spike S1RBD protein of the virus SARS-CoV-2.
  ACE2 Human, HEK293 Recombinant was coated on an ELISA plate at 1μg/ml overnight at 4 centigrade. Recombinant SARS-CoV-2 Spike Protein S1RBD was added (starting at a concentration of 500 ng/mL with a two-fold serial dilution) during one hour at RT and the interaction was then detected using an anti-human IgG (HRP).
• Molecular Mass: ~110kDa (SDS-PAGE)
  ~240kDa (Dimer, by SEC)
• Endotoxin: <0.01 EU/μg (LAL test).
• Purity: ≥ 95%
• Applications: Cell culture and/or animal studies, In vitro, COVID-19
• Storage: Stable for at least 6 months after receipt when stored at -20 centigrade. After opening, prepare aliquots and store at -20 centigrade. Working aliquots are stable for up to 3 months when stored at -20 centigrade. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.
• Storage Buffer: 1 mg/mL after reconstitution. Lyophilized. Contains PBS.
• Reconstitution: Reconstitute with 50 μL endotoxin-free water.
• Shipping: Shipped on ice packs.

Gene Information

• Gene Name: ACE2 angiotensin converting enzyme 2 [ Homo sapiens (human) ]
• Official Symbol: ACE2
• Synonyms: ACE2; angiotensin converting enzyme 2; ACEH; angiotensin-converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog; angiotensin-converting enzyme-related carboxypeptidase; metalloprotease MPROT15; peptidyl-dipeptidase A; EC 3.4.17.23
• Gene ID: 59272
• mRNA Refseq: NM_021804
• Protein Refseq: NP_068576
• MIM: 300335
• UniProt ID: Q9BYF1