Active Recombinant HIV-1 Integrase p31 Antigen, His-tagged

• Cat.No.: INT-356H
• Product Overview: Recombinant HIV-1 Integrase p31 Antigen (9-289 aa) is produced by E. coli expression system. The E.coli derived recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 immunodominant regions from the p31 protein (integrase) 9-289 amino acids, fused with six histidines at N-terminus.

Specification

• Species: HIV
• Source: E.coli
• Tag: His
• Protein Length: 9-289 aa
• Description: Integrase is an enzyme produced by the HIV which enables its genetic material to be integrated into the DNA of the infected cell and is a key component in the pre-integration complex. HIV integrase contains 3 domains, an N-terminal HH-CC zinc fingerdomainwhich is partially responsible for multimerization, a central catalytic domain and a C-terminal domain. Both Central catalytic domain and C-terminal domains have been shown to bind both viral and cellular DNA. No crystal structure data exists with Integrase bound to its DNA substrates. HIV-1 integrase functions as a dimeror a tetramer. Additionally, several host cellular proteins interact with integrase and may facilitate the integration process.
• Form: Sterile filtered colorless clear solution.. 1.5M urea, 25mM Tris-HCl pH 8.0, 0.2% Triton-X & 50% Glycerol.
• Bio-activity: Immunoreactive with all sera of HIV-1 infected individuals.
• Purity: > 95.0% as determined by HPLC analysis and SDS-PAGE.
• Applications: HIV-1 Integrase p31 antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
• Notes: LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
• Stability: HIV-1 Integrase p31 although stable at 4 centigrade for 1 week, should be stored below -18 centigrade.
  Please prevent freeze thaw cycles.