Active Recombinant H9N2 (A/Guinea fowl/Hong Kong/WF10/99) HA Protein, His & Avi-tagged, Biotinylated

• Cat.No.: HA-411I
• Product Overview: Biotinylated Recombinant H9N2 HA Protein (Asp 19 - Lys 523) (AAO46082.1), fused with an Avi tag at the C-terminus, followed by a polyhistidine tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: H9N2
• Tag: His&Avi
• Predicted N Terminal: Asp 19
• Form: Lyophilized from sterile PBS, pH7.4, 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Anti-Influenza HA Universal MAb CM2S3 at 5μg/mL (100 μL/well) can bind Biotinylated Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA with a linear range of 1.95-31.25 ng/mL.
• Molecular Mass: Recombinant H9N2 HA Protein has a calculated MW of 60 kDa. The protein migrates as 66-70 kDa on a SDS-PAGE gel under reducing (R) condition.
• Protein length: Asp 19 - Lys 523
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Official Symbol: HA