Active Recombinant H5N1 NA, His-tagged

• Cat.No.: NA-190O
• Product Overview: Influenza A virus (A/Thailand/1(KAN-1)/2004 (H5N1)) Neuraminidase (NA) (Influenza A virus (A/Thailand/1(KAN-1)/2004 (H5N1)) Neuraminidase (NA)) His 36 - Lys 449 (Accession # H8PF47) was produced in human 293 cells (HEK293).

Specification

• Species: H5N1
• Source: HEK293
• Tag: His
• Protein Length: 36-449 a.a.
• Description: Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 2"-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2"-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at
• Molecular Mass: Influenza A virus (A/Thailand/1(KAN-1)/2004 (H5N1)) Neuraminidase (NA) is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 46.1 kDa. The predicted N-terminus is His 36. DTT-reduced Protein migrates as 48 kDa in SDS-PAGE.
• Endotoxin: Less than 1.0 EU per μg of the Influenza A virus (A/Thailand/1(KAN-1)/2004 (H5N1)) Neuraminidase (NA) by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
• Reconstitution: See Certificate of Analysis for reconstitution instructions and specific concentrations.

Gene Information

• Gene Name: NA neuraminidase [ Influenza A virus (A/goose/Guangdong/1/1996(H5N1)) ]
• Official Symbol: NA
• Synonyms: NA, Neuraminidase, Influenza A, H5N1
• Gene ID: 3654619
• mRNA Refseq: NC_007361.1
• Protein Refseq: YP_308668.1