Active Recombinant Influenza A [A/Wisconsin/588/2019 (H1N1)] HA protein, His-tagged

• Cat.No.: HA-1542I
• Product Overview: Recombinant Influenza A [A/Wisconsin/588/2019 (H1N1)] HA protein(Asp18-Ile530), fused with His tag, was expressed in HEK293.

Specification

• Species: Influenza A [A/Wisconsin/588/2019 (H1N1)]
• Source: HEK293
• Tag: His
• Protein Length: 18-530 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.
• Bio-activity: Immobilized Influenza A [A/Wisconsin/588/2019 (H1N1)] HA, His Tag at 1 μg/mL (100 μL/well) can bind Human Anti-HA (H1N1) antibody 3E1 with a linear range of 0.2-4 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 63.1 kDa. The protein migrates as 75-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Asp18-Ile530
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.