Active Recombinant Full Length Rat Calpain 2 Protein, His-tagged

• Cat.No.: Capn2-2632R
• Product Overview: Active Recombinant Full Length Rat Calpain 2 Protein with His tag was expressed in E. coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: 1-700aa
• Description: Enables calcium ion binding activity; cytoskeletal protein binding activity; and enzyme binding activity. Contributes to calcium-dependent cysteine-type endopeptidase activity. Involved in several processes, including cellular response to interferon-beta; positive regulation of cell death; and positive regulation of myoblast fusion. Located in several cellular components, including external side of plasma membrane; focal adhesion; and neuronal cell body. Colocalizes with mitochondrial intermembrane space. Biomarker of hypertrophic cardiomyopathy and sciatic neuropathy. Orthologous to human CAPN2 (calpain 2).
• Tag: His
• Bio-activity: 1.47 U/mg protein. One unit is defined as the amount of enzyme that cleaves 1pmol of the caspase substrate N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin per 15 min at 37 centigrade.
• AA Sequence: MAGIAMKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLEAGALFQDPSFPALPSSLGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLDQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELRKPPPNLFKIIQKALEKGSLLGCSIDITSAADSEAVTYQKLVKGHAYSVTGAEEVESSGSLQKLIRIRNPWGQVEWTGKWNDNCPSWNTVDPEVRANLTERQEDGEFWMSFSDFLRHYSRLEICNLTPDTLTCDSYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDDEDGERGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELTGQTNIHLSKNFFLTTRARERSDTFINLREVLNRFKLPPGEYVLVPSTFEPHKNGDFCIRVFSEKKADYQTVDDEIEANIEEIEANEEDIGDGFRRLFAQLAGEDAEISAFELQTILRRVLAKREDIKSDGFSIETCKIMVDMLDEDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKLPCQLHQVIVARFADDELIIDFDNFVRCLVRLEILFKIFKQLDPENTGTIQLDLISWLSFSVLHHHHHHMHHHHHHHYSNIEANESEEERQFRKLFVQLAGDDMEVSATELMNILNKVVTRHPDLKTDGFGIDTCRSMVAVMDSDTTGKLGFEEFKYLWNNIKKWQGIYKRFDTDRSGTIGSNELPGAFEAAGFHLNQHIYSMIIRRYSDETGNMDFDNFISCLVRLDAMFRAFRSLDKNGTGQIQVNIQEWLQLTMYS
• Purity: >90% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 1.2 mg/mL by Bradford
• Storage Buffer: Sterile 50mM Tris, pH7.8, 100mM NaCl, 5mM EDTA, 5mM β-mercaptoethanol, 40% Glycerol

Gene Information

• Gene Name: Capn2 calpain 2 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Capn2
• Synonyms: Active Recombinant Full Length Rat Calpain 2 Protein, His-tagged; Capn2, His-tagged(Rat); Capn2-2632R; Capn2; calpain 2; calpain-2 catalytic subunit; CANP 2; M-calpain; calcium-activated neutral proteinase 2; calpain 2, (m/II) large subunit; calpain II; calpain M-type; calpain-2 large subunit; millimolar-calpain; EC 3.4.22.53
• Gene ID: 29154
• mRNA Refseq: NM_017116
• Protein Refseq: NP_058812
• UniProt ID: Q07009