Active Recombinant Full Length Human SPHK1 Protein, DDK-tagged, Biotinylated

• Cat.No.: SPHK1-1441HF
• Product Overview: Full-length human SPHK1 [1-384(end) amino acids of accession number NP_001136074.1] was expressed as N-terminal DYKDDDDK tagged, biotinylated protein (46 kDa) using baculovirus expression system. The protein was purified by using DYKDDDDK tag antibody agarose.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: Flag
• Protein Length: 1-384(end)
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Purity: 97%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: sphingosine kinase 1
• Official Symbol: SPHK1
• Synonyms: SPHK1;SPHK
• Protein Refseq: NP_001136074.1
• UniProt ID: Q9NYA1