Active Recombinant Full Length Human Protein Kinase C, Theta, GST-tagged

• Cat.No.: PRKCQ-408H
• Product Overview: Recombinant full-length human PRKCQ was expressed bybaculovirusinSf9 insect cellsusing an N-terminal GST tag. MW=110 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-706 a.a.
• Description: Protein Kinase C, theta (PKCθ) is important component in the intracellular signaling cascade. Recent studies have suggested that local accumulation of fat metabolites inside skeletal muscle may activate a serine kinase cascade involving PKC-theta leading to defects in insulin signaling and glucose transport in skeletal muscle. Insulin resistance plays a primary role in the development of type 2 diabetes and may be related to alterations in fat metabolism. PKC-theta is a crucial component mediating fat-induced insulin resistance in skeletal muscle and is a potential therapeutic target for the treatment of type 2 diabetes.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Bio-activity: 792 nmol/min/mg

Gene Information

• Gene Name: PRKCQ protein kinase C, theta [ Homo sapiens ]
• Synonyms: PRKCQ; protein kinase C, theta; PRKCT; MGC126514; MGC141919; nPKC-theta; Protein kinase C theta type; EC 2.7.11.13
• Gene ID: 5588
• mRNA Refseq: NM_006257
• Protein Refseq: NP_006248
• MIM: 600448
• UniProt ID: Q04759
• Chromosome Location: 10p15
• Pathway: Adipocytokine signaling pathway; T cell receptor signaling pathway; Tight junction; Vascular smooth muscle contraction; Apoptosis; Signaling in Immune system
• Function: ATP binding; calcium ion binding; nucleotide binding; protein kinase C activity; transferase activity; zinc ion binding