Active Recombinant Full Length Human CDK4 Protein, DDK-tagged, Biotinylated

• Cat.No.: CDK4-1621HF
• Product Overview: Full-length human CDK4 [1-303(end) amino acids of accession number NP_000066.1] was co-expressed as N-terminal DYKDDDDK tagged, biotinylated protein (37 kDa) with human GST-CyclinD3 [1-292(end) amino acids of accession number AAA51927.1] using baculovirus expression system. The protein was purified by using DYKDDDDK tag antibody agarose.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: Flag
• Protein Length: 1-303(end)
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
• Purity: 95%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: cyclin-dependent kinase 4
• Official Symbol: CDK4
• Synonyms: CDK4;CMM3;PSK-J3
• Protein Refseq: NP_000066.1
• UniProt ID: P11802