Active Recombinant E.coli zwf protein

• Cat.No.: zwf-001E
• Product Overview: Recombinant E.coli G7PD (1-491aa) protein without tag was expressed in E. coli.

Specification

• Species: E.coli
• Source: E.coli
• Protein Length: 1-491
• Description: Residues 199-203 of zwf encode a NNWDN peptide involved in ChpA(MazF) toxicity that is likely converted to NNWNN by AsnA; a zwf deletion prevents NNWNN activity (Kolodkin-Gal, 2007). [More information is available at EcoGene: EG11221]. A null mutation in the zwf gene encoding glucose 6-phosphate-1-dehydrogenase does not affect the growth rate significantly.
• Form: Liquid
• Bio-activity: Specific activity is >50 unit/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25 centigrade.
• Molecular Mass: 55.7 kDa
• AA Sequence: MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTRIIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRLGAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEINDQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVGIEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDRSNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFYLRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPGLDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVDSITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE
• Purity: >95% by SDS-PAGE
• Applications: Enzyme Activity, SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Bradford assay)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT

Gene Information

• Gene Name: zwf NADP(+)-dependent glucose-6-phosphate dehydrogenase [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: zwf
• Synonyms: zwf; NADP(+)-dependent glucose-6-phosphate dehydrogenase; ECK1853; NADP(+)-dependent glucose-6-phosphate dehydrogenase; EC 1.1.1.49
• Gene ID: 946370
• Protein Refseq: NP_416366
• UniProt ID: P0AC53