Active Recombinant E. coli RNase H

• Cat.No.: rnh-63E
• Product Overview: A recombinant?E. coli?strain carrying the RNAse H (rnh) gene from?E.coli.

Specification

• Species: E.coli
• Source: E.coli
• Tag: Non
• Description: E.coli?RNase H (rnh) is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestion produces ribonucleotide molecules with 5-phosphate and 3-hydroxyl termini. RNAse H is nearly inactive against single or double-stranded RNA molecules.
• Bio-activity: 625,000 U/mg
• Purity: >99% by SDS-PAGE
• Unit Definition: 1 unit is defined as the amount of enzyme that will hydrolyze 1 nmol of RNA from an 3H-labeled DNA:RNA hybrid molecule into acid-soluble material in 20 minutes at 37°C.
• Storage: -25 to -15°C
• Concentration: 5,000 U/ml
• Storage Buffer: Supplied in: 20 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol, pH 7.9 @ 25°C Supplied with: 10X RNAse H Buffer: 500mM Tris-HCl, 750 mM KCl, 30 mM MgCl2, 100 mM DTT, pH 8.3 @ 25?C.