Active Recombinant E.coli IdhA Protein, His-tagged

• Cat.No.: IdhA-391E
• Product Overview: Recombinant E.coli IdhA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D)-lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c.
• Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 100mM NaCl
• Bio-activity: Specific activity is >200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
• Molecular Mass: 39.1kDa (353aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ldhA fermentative D-lactate dehydrogenase, NAD-dependent [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: IdhA
• Synonyms: ECK1377; hslF; hslI; htpH; JW1375; fermentative D-lactate dehydrogenase, NAD-dependent
• Gene ID: 946315
• Protein Refseq: NP_415898
• UniProt ID: P52643
• Pathway: Microbial metabolism in diverse environments, organism-specific biosystem; Pyruvate metabolism, organism-specific biosystem; mixed acid fermentation, conserved biosystem