Active Recombinant E. coli glpK, His-tagged

• Cat.No.: glpK-259E
• Product Overview: Recombinant E. coli glpK (Accession # AAC76908) Met1-Glu502, fused with a C-terminal 6-His tag, was produced in E. coli-derived.

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• ProteinLength: 1-502 a.a.
• Predicted N Terminal: Thr2
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, Glycerol, Brij-35 and DTT.
• Bio-activity: Measured by its ability to transfer phosphate from ATP to glycerol.The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant E. coli glpK, His-tagged has a calculated MW of 57 kDa. In SDS-PAGE migrates as 52-55 kDa, reducing conditions.
• Purity: >75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: glpK glycerol kinase [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: glpK
• Synonyms: glpK; glycerol kinase; ECK3918; JW3897; glycerol kinase; NP_418361.1; EC 2.7.1.30
• Gene ID: 948423
• Protein Refseq: NP_418361