Active Recombinant Dog NTRK Protein, His-tagged, Alexa Fluor 647 conjugated

• Cat.No.: NTRK-1042CAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant canine NTRK (Met1-His430) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: Dog
• Source: HEK293
• Tag: His
• ProteinLength: 410
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized canine NTRK-His at 10 μg/mL (100 μL/well) can bind biotinylated mouse BDNF, The EC50 of biotinylated mouse BDNF is 0.07-0.17 μg/mL.
• Molecular Mass: The recombinant canine NTRK comprises 410 amino acids and has a predicted molecular mass of 45.6 kDa. The apparent molecular mass of the protein is approximately 66-76 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• N-terminal Sequence Analysis: Cys 32
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Synonyms: NTRK