Active Recombinant Dog CYP1A2 Protein

• Cat.No.: CYP1A2-119D
• Product Overview: Recombinant dog CYP1A2 protein and human CYP-reductase without tag were coexpressed in E. coli.

Specification

• Species: Dog
• Source: E.coli
• Description: Cytochrome P450 1A2 (CYP1A2) is one of the CYP450 mixed-function oxidase system that is of clinical importance due to the large number of drug interactions associated with its induction and inhibition.
• Bio-activity: Cytochrome c Reductase Activity: 235 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: 7-ethoxycoumarin O-deethylase
  Linear with time up to: 10 min(Determined at 1 µM substrate, using a P450 concentration of 2.5 pmol/ml)
  Linear with P450 up to: 10 pmol/ml(Determined at 1 µM substrate, using an incubation time of 5 min)
  Vmax: 1.3 pmol/min/pmol CYP
  Km: 1.1 μM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 12.2 nmol/ml
  Protein Concentration: 10.0 mg/ml
  Specific P450 Content: 1220 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP1A2 cytochrome P450 family 1 subfamily A member 2 [ Canis lupus familiaris (dog) ]
• Official Symbol: CYP1A2
• Synonyms: CYP1A2; cytochrome P450 family 1 subfamily A member 2; DAH2; CYPIA2; cytochrome P450 1A2; Cholesterol 25-hydroxylase; Cytochrome P450-D2; Cytochrome-P450-1A2; cytochrome P450, family 1, subfamily A, polypeptide 2; hydroperoxy icosatetraenoate dehydratase
• Gene ID: 494010
• mRNA Refseq: NM_001008720
• Protein Refseq: NP_001008720
• UniProt ID: Q5R2U2