Active Recombinant Danio rerio (zebrafish) BMP-2 Protein

Cat.No. : BMP2A-299D
Product Overview : Recombinant Danio rerio (zebrafish) BMP2A (B3DI86) (Gln272-Arg386) was expressed and purified with an initial Met.
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Species : zebrafish
Source : E.coli
Tag : Non
ProteinLength : Gln272-Arg386
Description : BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.
Predicted N Terminal : Met
Form : Lyophilized from sterile 30mM HAC, pH 3.0. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.5-3 μg/mL.
Molecular Mass : The recombinant zebrafish BMP2A consists of 116 amino acids and has a calculated molecular mass of 13.2 kDa. It migrates as an 13 KDa band in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 95 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Official Symbol BMP2A
Synonyms BMP2A;bone morphogenetic protein 2

Not For Human Consumption!

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