Active Recombinant Cynomolgus TNFSF8, His tagged

• Cat.No.: TNFSF8-68C
• Product Overview: Recombinant Cynomolgus TNFSF8 (G7P2F1) (Gln63-Asp234), fused with a polyhistide tag at the N-terminus, was produced in Human Cells.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: Gln63-Asp234
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Immobilized Cynomolgus His-TNFSF8 at 10 μg/ml (100 μl/well) can bind biotinylated human CD30-Fch, The EC50 of biotinylated human CD30-Fch is 2.7-6.2 ng/ml.
• Molecular Mass: The recombinant heterodimer of cynomolgus TNFSF8 comprises 192 amino acids and has a calculated molecular mass of 22 KDa. The apparent molecular mass of the protein is approximately 38 KDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.