Active Recombinant Cynomolgus TNFSF13 protein, His-Flag-tagged

• Cat.No.: TNFSF13-2884C
• Product Overview: Recombinant Cynomolgus TNFSF13 protein(Gln 111-Leu 250), fused with His and Flag tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Flag&His
• Protein Length: Gln 111-Leu 250
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus APRIL, His,Flag Tag at 1 μg/mL (100 μL/well) can bind Human TACI (R119A, R122A), Fc Tag with a linear range of 2-13 ng/mL.Immobilized Cynomolgus APRIL, His,Flag Tag at 1 μg/mL (100 μL/well) can bind Human BCMA, Fc Tag (HPLC verified) with a linear range of 0.1-1 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.The protein has a calculated MW of 50.4 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.