Active Recombinant Cynomolgus TNFRSF17 protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: TNFRSF17-1827C
• Product Overview: Biotinylated Cynomolgus BCMA, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 53 (Accession # XP_001106892.1). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: AA Met 1 - Ala 53
• Description: Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.
• Predicted N Terminal: Met 1
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human BAFF, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus BCMA, Fc Tag with a linear range of 0.2-4 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 33.9 kDa. The protein migrates as 42-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: TNFRSF17 TNF receptor superfamily member 17 [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: TNFRSF17
• Synonyms: BCMA; tumor necrosis factor receptor superfamily, member 17
• Gene ID: 102145399
• mRNA Refseq: XM_005591286.2
• Protein Refseq: XP_005591343.1
• UniProt ID: G7Q0I4