Active Recombinant Cynomolgus TEK protein, hFc-tagged

• Cat.No.: TEK-212C
• Product Overview: Recombinant Cynomolgus TEK protein(G7PS68)(Met1-Lys745), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: Fc
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse S1h-3C-mANGPT2 (Cat:50298-M07H) at 10 μg/ml (100 μl/well) can bind Cynomolgus TEK-Fc, The EC50 of Cynomolgus TEK-Fc is 0.7-1.64 μg/ml.
• Molecular Mass: The recombinant cynomolgus TEK is a disulfide-linked homodimer. The reduced monomer comprises 964 amino acids and has a calculated molecular mass of 107.6 KDa.
• Protein length: Met1-Lys745
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.