Active Recombinant Cynomolgus SIRPA protein, His-tagged

• Cat.No.: SIRPA-3654C
• Product Overview: Recombinant Cynomolgus SIRPA protein(XP_015313153.1) (Met1-Tyr372) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized cynoSIRPAh at 10 μg/mL (100 μL/well) can bind CD47-Fc, the EC50 of CD47-Fc is 3.0-10.0 ng/mL.
• Molecular Mass: The recombinant cynomolgus SIRPA comprises 353 amino acids and has a calculated molecular mass of 38.93 kDa.
• Protein length: Met1-Tyr372
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: >95% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.

Gene Information

• Gene Name: SIRPA signal regulatory protein alpha [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: SIRPA
• Synonyms: SIRPA
• Gene ID: 101926317
• mRNA Refseq: NM_001284750
• Protein Refseq: NP_001271679