Active Recombinant Cynomolgus/Rhesus ANGPT2 protein, His-tagged

• Cat.No.: ANGPT2-2167C
• Product Overview: Recombinant Cynomolgus/Rhesus ANGPT2 protein(XP_005562586.1 & XP_001097949.1)(Lys274-Phe495), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus/Rhesus
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus Angiopoietin-2 His at 2 μg/ml (100 μl/well) can bind Cynomolgus TIE2-hFc, the EC50 of Cynomolgus TIE2-hFc is 15-90 ng/mL.
• Molecular Mass: The recombinant cynomolgus / rhesus ANGPT2 consists 241 amino acids and predicts a molecular mass of 27.7 kDa.
• Protein length: Lys274-Phe495
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.