Active Recombinant Cynomolgus RBP4, His tagged

• Cat.No.: RBP4-112C
• Product Overview: Recombinant Cynomolgus RBP4 (Met1-Leu201), fused with a polyhistidine tag at the C-terminus, was produced in Human Cells.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: Met1-Leu201
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind alltrans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4.
  The 50% binding concentration (BC50) is >1 µM.
• Molecular Mass: The recombinant cynomolgus RBP4 comprises 194 amino acids and has a calculated molecular mass of 22.6 KDa. The apparent molecular mass of it is approximately 23 KDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.