Active Recombinant Cynomolgus IL2RA protein, His-tagged

• Cat.No.: IL2RA-1631C
• Product Overview: Recombinant Cynomolgus IL2RA protein(Glu22-Glu238), fused with His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 22-238 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Cynomolgus IL-2 R alpha / CD25, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2 Protein, Fc,Avitag with a linear range of 1-20 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 26.3 kDa. The protein migrates as 52-57 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu22-Glu238
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.