Active Recombinant Cynomolgus IGFBP5 protein, His-tagged

• Cat.No.: IGFBP5-065C
• Product Overview: Recombinant Cynomolgus IGFBP5 protein (F1PEQ5) (Met1-Glu271) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: Met1-Glu271
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. The ED50 for this effect is 1-6 μg/mL in the presence of 14 ng/mL rhIGF-II.
• Molecular Mass: The recombinant cynomolgus IGFBP5 comprises 263 amino acids and has a calculated molecular mass of 30 KDa. The apparent molecular mass of it is approximately 38 and 23 KDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: >90% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.