Active Recombinant Cynomolgus IFNA8 protein, mFc-tagged

• Cat.No.: IFNA8-2195C
• Product Overview: Recombinant Cynomolgus IFNA8 protein(G7PS89)(Met1-Glu189), fused with mFc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: mFc
• ProteinLength: Met1-Glu189
• Form: Lyophilized from sterile PBS, PH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in antiviral assay using WISH human amnion cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 0.1-0.6 ng/mL.
• Molecular Mass: The recombinant cynomolgus IFNA8 is a disulfide-linked homodimer. The reduced monomer comprises 400 amino acids and has a calculated molecular mass of 46 KDa.The apparent molecular mass of the protein is approximately 48 KDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.