Active Recombinant Cynomolgus IFNA8, His tagged

• Cat.No.: IFNA8-74C
• Product Overview: Recombinant Cynomolgus IFNA8 (G7PS89) (Met1-Glu189), fused with a polyhistidine tag at the C-terminus, was produced in Human Cells.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: Met1-Glu189
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus.
  The ED50 for this effect is typically 1-5 pg/mL.
• Molecular Mass: The recombinant cynomolgus IFNA8 comprises 177 amino acids and has a calculated molecular mass of 21 KDa. The apparent molecular mass of it is approximately 33 KDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.