Active Recombinant Cynomolgus HAVCR2 protein, Fc-tagged

• Cat.No.: HAVCR2-0761C
• Product Overview: Recombinant Cynomolgus HAVCR2 protein(Ser22-Arg201), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc
• Protein Length: 22-201 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus TIM-3, Fc Tag at 2μg/mL (100 μL/well) can bind Anti-TIM3 Mab, Human IgG4 with a linear range of 0.5-4 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 45.8 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ser22-Arg201
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >85% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.