Active Recombinant Cynomolgus GPRC5D protein, His&Flag-tagged

• Cat.No.: GPRC5D-508H
• Product Overview: Recombinant Cynomolgus GPRC5D protein(A0A2K5W6I7-1)(Tyr 2 - Phe 192 & Glu 197 - Leu 262), fused with C-terminal His tag and N-terminal Flag tag, was expressed from HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Flag&His
• Protein Length: Tyr 2 - Phe 192 & Glu 197 - Leu 262
• Form: Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
• Bio-activity: Anti-GPRC5D antibody immobilized on CM5 Chip can bind Cynomolgus GPRC5D, Flag,His Tag (Nanodisc) (Cat. No. GPD-C82D4) with an affinity constant of 0.134 μM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: The protein has a calculated MW of 51.9 kDa.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >80% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.