Active Recombinant Cynomolgus GDF15 protein, His-tagged

• Cat.No.: GDF15-1483C
• Product Overview: Recombinant Cynomolgus GDF15 protein(Ala197-Val308), fused with His tag, was expressed in E.coli .

Specification

• Source: E.coli
• Species: Cynomolgus
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 0.085% TFA in 30% ACN with trehalose as protectant.
• Bio-activity: Immobilized Cynomolgus GDF-15, His Tag at 1 μg/mL (100 μL/well) can bind Human GFR alpha-like, Fc Tag with a linear range of 0.8-25 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 14.4 kDa. The protein migrates as 16 kDa under reducing (R) condition (SDS-PAGE).
• Proteinlength: Ala197-Val308
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 197-308 a.a.