Active Recombinant Cynomolgus FCGR2B protein, His/Avi-tagged, Biotinylated

• Cat.No.: FCGR2B-291C
• Product Overview: Biotinylated Recombinant Cynomolgus FCGR2B protein (AAL92097.1) extracellular domain (Met 1-Gly 220) was expressed in HEK293, fused with a c-terminal polyhistidine tagged Avi tag at the C-terminus.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&His
• Protein Length: Met 1-Gly 220
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Cynomolgus CD32B/Fcgr2b Protein (His & AVI Tag), Biotinylated at 2 μg/mL (100 μL/well) can bind Human IgG2 Protein (257 Ser/Ala), the EC50 is 4-36 ng/mL.
• Molecular Mass: The recombinant cynomolgus CD32b consists of 211 amino acids and has a calculated molecular mass of 23.5 kDa. The apparent molecular mass of the recombinant protein is approximately 34 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.