Active Recombinant Cynomolgus FCER1A protein, Fc-tagged

• Cat.No.: FCER1A-1459C
• Product Overview: Recombinant Cynomolgus FCER1A protein(Val29-Lys204), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc
• Protein Length: 29-204 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5.
• Bio-activity: Loaded Cynomolgus Fc epsilon RI alpha, Fc Tag on AHC Biosensor, can bind Immunoglobulin E, Human Plasma with an affinity constant of 1.65 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 46.9 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Val29-Lys204
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.