Active Recombinant Cynomolgus CYP3A4 Protein

• Cat.No.: CYP3A4-128C
• Product Overview: Recombinant cynomolgus CYP3A4 protein and human CYP-reductase without tag were coexpressed in E. coli.

Specification

• Description: Catalyzes nifedipine and nilvadipine oxidations.
• Source: E. coli
• Species: Cynomolgus
• Bio-activity: Cytochrome c Reductase Activity: 171 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Testosterone 6β-hydroxylase
  Linear with time up to: 15 min(Determined at 15 µM substrate, using a P450 concentration of 25 pmol/ml)
  Linear with P450 up to: 60 pmol/ml(Determined at 15 µM substrate, using an incubation time of 5 min)
  Vmax: 4.8 pmol/min/pmol CYP
  Km: 17 μM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 5.0 nmol/ml
  Protein Concentration: 11.7 mg/ml
  Specific P450 Content: 427 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP3A4 cytochrome P450 family 3 subfamily A member 4 [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: CYP3A4
• Synonyms: CYP3A4; cytochrome P450 family 3 subfamily A member 4; CYP3A8; CYPIIIA8; cytochrome P450 3A8; Cytochrome P-450-MK2; Cytochrome P450 3A8; Cytochrome P450-MKNF2; P-450 3A; cytochrome P-450 3A
• Gene ID: 102144258
• mRNA Refseq: NM_001284534
• Protein Refseq: NP_001271463
• UniProt ID: P33268