Active Recombinant Cynomolgus CD40 protein, His-tagged

• Cat.No.: CD40-23C
• Product Overview: Recombinant Cynomolgus CD40 protein(Glu 21 - Arg 193), fused with His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: Glu 21 - Arg 193
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus CD40, His Tag at 5 μg/mL (100 μL/well) can bind Mouse CD40 Ligand, Fc Tag with a linear range of 1-6.6 ng/mL.Immobilized Cynomolgus CD40, His Tag at 5 μg/mL (100 μL/well) can bind Human / Rhesus macaque CD40 Ligand, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.3-5 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 21.1 kDa. The protein migrates as 26-32 kDa under reducing (R) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.