Active Recombinant COVID-19 Spike protein S1 subunit, His-tagged

• Cat.No.: Spike-195V
• Product Overview: Recombinant COVID-19 (2019 novel coronavirus) Spike protein S1 subunit was fused to His-tag at C-terminus and expressed in Baculovirus-Insect cells.

Specification

• Source: Insect Cells
• Species: COVID-19
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag), the EC50 of SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag) is 300-700 ng/mL.
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His tag) consists of 681 amino acids and predicts a molecular mass of 76.45 kDa.
• Protein length: Val16-Arg685
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.