Active Recombinant COVID-19 Spike Trimer protein(BA.4/Omicron), His-Avi-tagged, Biotinylated

• Cat.No.: Spike-713V
• Product Overview: Biotinylated Recombinant COVID-19 Spike Trimer protein(BA.4/Omicron)(Val 16 - Pro 1213)(Accession # QHD43416.1(T19I, LPP24-26del, A27S, HV69-70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N658S, N679K, P681H, N764K, D796Y, Q954H, N969K, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)), fused with His and Avi tag at the C-terminus, was expressed in HEK293.

Specification

• Source: HEK293
• Species: COVID-19
• Tag: His&Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (BA.4/Omicron) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag with a linear range of 0.001-0.013 μg/mL.
• Molecular Mass: The protein has a calculated MW of 139.4 kDa. The protein migrates as 160-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Val 16 - Pro 1213
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
  >90% as determined by SEC-MALS.
• Storage: This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: Spike
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2