Active Recombinant COVID-19 Spike Protein (C136F, Y144del, R190S, D215G, LA242-243del, Y449H, E484K, N501Y, D614G, H655Y, N679K, T716I, T859N), His-Avi-tagged, Biotinylated

• Cat.No.: Spike-4817V
• Product Overview: Biotinylated Recombinant COVID-19 Protein (C136F, Y144del, R190S, D215G, LA242-243del, Y449H, E484K, N501Y, D614G, H655Y, N679K, T716I, T859N)(Val 16 - Pro 1213), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: COVID-19
• Tag: His&Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag at 5 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag with a linear range of 0.1-3 ng/mL.Immobilized Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag at 5 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.2-6 ng/mL.Immobilized Human ACE2, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag with a linear range of 2-39 ng/mL.Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag with a linear range of 0.001-0.05 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 139.5 kDa. The protein migrates as 180-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Val 16 - Pro 1213
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: Spike
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2