Active Recombinant COVID-19 Spike S Trimer protein, His-Avi-tagged, Biotinylated

• Cat.No.: Spike-010V
• Product Overview: Biotinylated Active Recombinant COVID-19 Spike S Trimer protein(QHD43416.1)(Val16-Glu1188), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: COVID-19
• Source: HEK293
• Tag: Avi&His
• Protein Length: Val16-Glu1188
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated SARS-COV-2 Spike S (Trimer) , His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 136.6 kDa. Due to glycosylation, the protein migrates to 140-170 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.