Active Recombinant COVID-19 N protein, His-tagged

• Cat.No.: N-465S
• Product Overview: Recombinant COVID-19 N protein(Met 1 - Ala 419), fused with His tag, was expressed in HEK293.

Specification

• Species: Sars-CoV-2
• Source: HEK293
• Tag: His
• Protein Length: Met 1 - Ala 419
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 0.2M Arginine, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (BQ.1/Omicron) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.1-2 ng/mL. Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (BQ.1/Omicron) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL.
• Molecular Mass: Molecular CharacterizationThis protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 47.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.