Active Recombinant COVID-19 N protein(BA.3*(S33V)/Omicron), His-tagged

• Cat.No.: N-730V
• Product Overview: Recombinant COVID-19 N protein(BA.3*(S33V)/Omicron)(Met 1 - Ala 419)(Accession # QHO62115.1(P13L, GER 30-32del, S33V, R203K, G204R, S413R)), fused with His tag at the C-terminus, was expressed in HEK293.

Specification

• Species: COVID-19
• Source: HEK293
• Tag: His
• ProteinLength: Met 1 - Ala 419
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 0.2M Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (BA.3*(S33V)/Omicron) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.5-2 ng/mL.
• Molecular Mass: The protein has a calculated MW of 47.1 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: N nucleocapsid phosphoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: N
• Synonyms: coronavirus NP Protein, 2019-nCoV; coronavirus Nucleocapsid Protein, 2019-nCoV; coronavirus Nucleoprotein Protein, 2019-nCoV; cov np Protein, 2019-nCoV; ncov NP Protein, 2019-nCoV; NCP-CoV Nucleocapsid Protein, 2019-nCoV; novel coronavirus NP Protein, 2019-nCoV; novel coronavirus Nucleocapsid Protein, 2019-nCoV; novel coronavirus Nucleoprotein Protein, 2019-nCoV; np Protein, 2019-nCoV; nucleocapsid Protein, 2019-nCoV; Nucleoprotein Protein, 2019-nCoV
• Gene ID: 43740575
• Protein Refseq: YP_009724397.2
• UniProt ID: P0DTC9