Active Recombinant Colony Stimulating Factor 2 (Granulocyte-Macrophage)

• Cat.No.: CSF2-648C
• Product Overview: GMCSF Canine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14.2 kDa. GM-CSF is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: Canine
• Tag: Non
• Description: GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
• Form: GMCSF was lyophilized after extensive dialysis against 1x PBS pH= 7.4μ0.1.
• Purity: Greater than 96.0% as determined by 1. Analysis by RP-HPLC. 2. Analysis by SDS-PAGE.
• Bio-activity: The ED50 as calculated by the dose-dependant stimulation of the proliferation of human TF1 erythroleukemic cells is typically 1-4 ng/ml.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized GMCSF in sterile deionized water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Amino acid sequence: APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPE GPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQN INFKSFKENL KDFLFNIPFD CWKPVKK.
• Storage: It is recommended to reconstitute the lyophilized GMCSF in sterile 18Mμ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: CSF2 colony stimulating factor 2 (granulocyte-macrophage) [ Canis lupus familiaris ]
• Official Symbol: CSF2
• Synonyms: CSF2; GM-CS; granulocyte-macrophage colony-stimulating factor; CSF; colony-stimulating factor
• Gene ID: 403923
• mRNA Refseq: NM_001003245
• Protein Refseq: NP_001003245
• UniProt ID: P48749
• Pathway: Amoebiasis; Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; HTLV-I infection; Hematopoietic cell lineage; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; Rheumatoid arthritis; T cell receptor signaling pathway