Active Recombinant Canine TNF protein, His-tagged

• Cat.No.: TNF-1609C
• Product Overview: Recombinant Canine TNF protein(Ser22-Thr338), fused with His tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: His
• Protein Length: 22-338 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Immobilized Canine IL-13 R alpha 2, His Tag at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-IL-13 R alpha 2 Antibody, Mouse IgG1 with a linear range of 0.2-2 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 40.3 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ser22-Thr338
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by reduced SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.