Active Recombinant Canine PDCD1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: PDCD1-0610C
• Product Overview: Biotinylated Recombinant Canine PDCD1 protein(Leu25-Gly168), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: Avi&His
• Protein Length: 25-168 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human PD-L1, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Canine PD-1, His,Avitag with a linear range of 0.001-0.125 μg/mL. Immobilized Biotinylated Canine PD-1, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human PD-1 Antibody, Human IgG4 with a linear range of 0.004-1 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 19.7 kDa. The protein migrates as 32-36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu25-Gly168
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.