Active Recombinant Canine IL33 protein

• Cat.No.: IL33-1034C
• Product Overview: Recombinant Canine IL33 protein(O97863)(Ser110-Ser263) was expressed in E. coli.

Specification

• Species: Canine
• Source: E.coli
• Tag: Non
• Protein Length: Ser110-Ser263
• Form: Lyophilized from sterile PBS, pH7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Canine IL33 at 10 μg/ml (100 μl/well) can bind human IL1R4-Fc, The EC50 of human IL1R4-Fc is 0.25-0.66 μg/ml.
• Molecular Mass: The recombinant canine IL33 consists of 155 amino acids and has a calculated molecular mass of 17.8 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 19KDa band.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.